Cultrex® In Vivo Vascular Permeability Assay Kit Instructions

The vascular endothelium is a thin, monolayer of cells that provide access to the exchange of biological materials such as gases, nutrients and metabolic waste. The endothelial layer forms a selective barrier between the vascular lumen and the surrounding tissue that controls this exchange. Under normal circumstances, endothelial cells exert a barrier function by strictly restricting the passage of biological macromolecules in the blood through structures such as intercellular junctions; however, in some physiological or pathological conditions, barrier function is impaired, and permeability can be greatly increased. To allow biomacromolecules in the blood to pass through and enter the subendothelial tissue or tissue space. There are many vasoactive cytokines and growth factors that regulate vascular permeability, such as interleukin-1α and β1, TNF-α2, IFN-γ, HGF 3 and VEGF4; some serious systemic diseases such as cancer, diabetes, heart Disease, shock, high blood pressure and arthritis are associated with vascular permeability collapse.

As a global leader in cell behavioral analysis, Trevigen offers complete experimental solutions for angiogenesis and vascular permeability analysis. Among them, the in vitro angiogenesis assay kit and the in vitro angiogenesis endothelial cell infiltration assay kit evaluate the angiogenic factors at the cellular level, respectively, and the patented DIVAA product is the world's first for quantitative detection of angiogenesis in vivo. Detection system. The Trevigen Cultrex In Vitro Vascular Permeability Assay Kit provides a solution for rapid and high-throughput screening of vascular permeability-related compounds in screening signal transduction pathways.

The Cultrex® In Vivo Vascular Permeability Assay Kit provides a flexible, standardized, high-throughput protocol to quantify the extent to which proteins or compounds affect the maintenance of intercellular adhesions in endothelial cells. The separate 24-well plate style and 96-well plate style available at the same time can be adapted to individual experiments or large-scale screening. This kit uses a simplified chemotactic chamber designed with an 8 μm PET film. The small holes in the migration chamber (top) can lead to the analysis chamber (bottom) without disassembly. This design is easy to operate, prevents contamination, and is suitable for mechanized high-throughput operating systems. The analysis chamber can be analyzed directly on a 96-well plate, reducing the experimental influencing factors introduced between the steps. Quantitative detection of vascular permeability was performed using FITC-labeled dextran (Dextran) as a permeability assay. Continuous monolayer cells form a barrier through intercellular adhesion mechanisms such as attachment (adhesion, tight junctions, and gap junctions). This barrier strictly controls FITC-dextran in the top chamber. Damage to the intercellular adhesion mechanism creates voids in the extracellular space, which allows FITC-dextran to enter the bottom chamber. The resulting diffuse FITC-dextran is associated with the number of extracellular voids, which embodies the permeability of the blood vessels.

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