Tissue culture generally uses MS medium. In the preparation, a large number of elements, trace elements, vitamins, and iron salts are first formulated into a mother liquor. Then, the mother liquor is sequentially mixed with a pipette or a graduated cylinder and mixed together, and added to the dissolved agar and sucrose solution. After adding the required amount of water and hormones, the mother liquor can be dispensed into a test tube or a flask or a culture bottle. After use.
(a) a large number of elements of the mother liquor preparation of potassium nitrate KNO3 19000 mg of magnesium sulfate MgSO47H2O 3700 mg of ammonium phosphate NH4NO3 16500 mg of potassium dihydrogen phosphate KH2PO4 1700 mg of calcium chloride CaCl22H2O 4400 mg of distilled water 1000 ml Preparation of drugs should pay attention to the order, otherwise due to calcium ions When dissolved together with phosphate, it reacts to produce a precipitate. Therefore, the first four kinds of solutions should be mixed with water and then added to a certain amount, and then calcium chloride solution is added, and finally the amount of water is added to 1000 ml.
(B) Preparation of Trace Elements Mother Liquor Manganese Sulfate MnSO44H2O 2230 mg Zinc Sulfate ZnSO47H2O 860 mg Boric Acid H3BO3 620 mg Potassium Iodide KI 83 mg Sodium Molybdate Na2MoO42H2O 25 mg Copper Sulphate CuSO45H2O 2.5 mg Cobalt Chloride CoCl26H2O 2.5 mg Water H2O 1000 mg Separate the above drugs First mix with 100 times liquid and then mix. Finally, add distilled water to 1000 ml. Serve.
(c) Vitamin mother liquor Glycine 100 mg Thiamine hydrochloride 20 mg Pyridoxine hydrochloride 25 mg Niacin 25 mg Inositol 5000 mg Water 500 ml This is 100 times liquid after mixing, that is, each ingredient is dissolved and mixed with distilled water to quantify to 500 Ml.
(d) Iron salt mother liquor
Na-EDTA 3730 mg ferrous sulfate FeSO47H2O 2780 mg H2O 500 ml, respectively, dissolved and mixed with distilled water to quantify to 500 ml, when preparing the medium, add 5 ml of this liquid per 1000 ml of medium.
Sucrose is used at a rate of 30 g of agar per 1000 ml plus 10 g of somatomedin per 1,000 ml. Tissue culture requires the addition of one or two growth regulators to the medium to increase callus formation. The growth of new plants. The rate of subculture and the role of root growth. Plant growth regulators include auxins such as 2,4D, naphthaleneacetic acid (NAA), indole acetic acid (IAA), indolebutyric acid (IBA) and the like; cytokinins such as kinetin (KT), 6-benzylaminopurine (BA), zeatin (ZT), isopentenyl purine (2iP), etc.; gibberellin (G3); dwarfism (P333).
The amount of auxin added is generally very small and it is necessary to prepare a certain concentration of mother liquor. The preparation of the mother liquor needs to select the appropriate solvent, such as 2,4-D, NAA, IAA and IBA are alcohol-soluble, so first dissolve with a small amount of 95% alcohol and then dilute with distilled water to a certain concentration. Cytokinins such as KT, BA, 2iP, and ZA need to be first dissolved in 1 molar hydrochloric acid or sodium hydroxide and then diluted with distilled water to a certain concentration and then stored in the refrigerator for later use. The preparation of folic acid mother liquor is first dissolved with a small amount of dilute ammonia, and then diluted with double distilled water. The temperature of the refrigerator is controlled at 5-8 degrees Celsius. However, no matter which auxin mother liquor is stored in the refrigerator, it cannot be stored for a long time. Once precipitation or growth of mold occurs in the mother liquor, it cannot be reused and must be reconstituted.
Several common medium composition components and amounts used in tissue culture of asparagus are listed in Table 9. The actual operation can also be added to improve.

Most products of Fluorine-Containing Benzaldehyde are a pale yellow liquid. They are playing an important role in fine chemical intermediates, is used as the main raw material for the synthesis of a variety of pesticides, pharmaceuticals, surfactants, polymer monomer mildew. As used in medicine synthesis antihypertensive drugs, antipyretic analgesic anti-inflammatory drugs, anti-cancer drugs, muscle relaxation drugs for synthesizing fluorine cypermethrin, fluorine chlorobenzene daisy cool in pesticides, fluoride and styrene, wherein the polymer of styrene monomer to fluorine can be widely used in the manufacture of the recording material, a thermoplastic resin coating, liquid crystal materials. Currently new miticide is effective pesticide products which is the main raw material of Fluorine-Containing Benzaldehyde.


Fluorine-Containing Benzaldehyde

Containing Benzaldehyde,5-Dichlorobenzaldehyde,3-Bromo-5-Fluorobenzaldehyde

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