Under the support of special projects of the non-profitable agriculture industry and the National 863 Program, the rapid detection technology for aquatic animal pathogens at the Yellow Sea Institute of the Chinese Academy of Water Sciences has made a series of progress and achieved remarkable results.
Researchers at the Yellow Sea Disease Research Institute applied loop-mediated isothermal amplification (LAMP) technology to the detection of aquatic animal pathogens, and developed complementary cores for the rapid preparation of nucleic acids, long-term preservation of reagents at room temperature, and immobilization of nucleic acid dyes. The technology solves a series of key technical problems in the practical application of rapid pathogen detection techniques for aquatic animals. White shrimp syndrome virus (WSSV), taura syndrome virus (TSV), infectious subcutaneous and hematopoietic necrosis virus (IHHNV), hepato-pancreatic parvovirus (HPV), P. monodon baculovirus (MBV) have been developed so far. ), Yellowhead virus (YHV), Infectious muscle necrosis virus (IMNV), Macrobrachium rosenbergii (MrNV), Turbot disease iridescent virus (TRBIV), Scallop acute viral necrosis virus (AVNV), A series of on-site rapid and highly sensitive detection techniques and test kits for pathogenic Edwardsiella. Using this series of detection technology and test kits, only a simple heat preservation device such as a thermos cup and thermometer can be used to detect aquatic animal pathogens in 50-60 minutes, and the test results are easy to determine; the sensitivity of the kit is very high. , can detect as low as 101 copies of the virus; compared with other detection technologies and test kits, the technology system detection method is low cost, the entire process does not involve toxic reagents, and is very safe for the operator and the environment.
In recent years, the institute has provided hundreds of shrimp virus detection kits to scientific research institutes and production units, and its application has shown good response. Combining project research and demonstration work, researchers conducted technical training on the use of kits for the technical backbones of farmers demonstration units in many provinces and cities in China's major shrimp aquaculture areas and received unanimous approval from trainees. At present, the technology has applied for 10 national invention patents, authorized 4 items, and published 2 SCI articles.
The series of detection technologies and kits have the advantages of good practicability, high specificity, high sensitivity, convenience, rapidity, and environmental friendliness, and are very suitable for popularization and application in grassroots production enterprises or units. In order to improve the level of aquatic animal disease diagnosis, rapid quarantine and epidemic monitoring in China, it provides a good technical guarantee.
Researchers at the Yellow Sea Disease Research Institute applied loop-mediated isothermal amplification (LAMP) technology to the detection of aquatic animal pathogens, and developed complementary cores for the rapid preparation of nucleic acids, long-term preservation of reagents at room temperature, and immobilization of nucleic acid dyes. The technology solves a series of key technical problems in the practical application of rapid pathogen detection techniques for aquatic animals. White shrimp syndrome virus (WSSV), taura syndrome virus (TSV), infectious subcutaneous and hematopoietic necrosis virus (IHHNV), hepato-pancreatic parvovirus (HPV), P. monodon baculovirus (MBV) have been developed so far. ), Yellowhead virus (YHV), Infectious muscle necrosis virus (IMNV), Macrobrachium rosenbergii (MrNV), Turbot disease iridescent virus (TRBIV), Scallop acute viral necrosis virus (AVNV), A series of on-site rapid and highly sensitive detection techniques and test kits for pathogenic Edwardsiella. Using this series of detection technology and test kits, only a simple heat preservation device such as a thermos cup and thermometer can be used to detect aquatic animal pathogens in 50-60 minutes, and the test results are easy to determine; the sensitivity of the kit is very high. , can detect as low as 101 copies of the virus; compared with other detection technologies and test kits, the technology system detection method is low cost, the entire process does not involve toxic reagents, and is very safe for the operator and the environment.
In recent years, the institute has provided hundreds of shrimp virus detection kits to scientific research institutes and production units, and its application has shown good response. Combining project research and demonstration work, researchers conducted technical training on the use of kits for the technical backbones of farmers demonstration units in many provinces and cities in China's major shrimp aquaculture areas and received unanimous approval from trainees. At present, the technology has applied for 10 national invention patents, authorized 4 items, and published 2 SCI articles.
The series of detection technologies and kits have the advantages of good practicability, high specificity, high sensitivity, convenience, rapidity, and environmental friendliness, and are very suitable for popularization and application in grassroots production enterprises or units. In order to improve the level of aquatic animal disease diagnosis, rapid quarantine and epidemic monitoring in China, it provides a good technical guarantee.
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