For some samples that are particularly hard or tough, such as: hard bones, cartilage, nails, scales, connective tissue, seeds, etc., it is difficult to ensure the grinding and crushing effect by using ordinary mechanical grinding methods to ensure that the sample is always at a low temperature. Although the conventional manual grinding method can take care of both, when the number of samples is relatively large, manual grinding is very time consuming and laborious, and there is often a possibility of cross contamination. If it is necessary to extract substances sensitive to temperature such as RNA and enzymes, it is very important to use liquid nitrogen for pretreatment to ensure that the sample is always at a low temperature during the crushing process.

When Bullet Blender is used to break up tissue cells, the sample is pre-frozen with liquid nitrogen, which ensures the efficiency of the sample and ensures that the sample is always at a low temperature. Take bones and plant leaves as an example. The specific operation methods are as follows:

skeleton:

Take 0.3 g of bone, use the filter paper to absorb the surface moisture of the bone, put it into the sample tube, add 5 pieces of 1.6mm stainless steel grinding beads, add 3 scoops of 0.5mm stainless steel grinding beads, and cover the tube cover. The entire tube was frozen in liquid nitrogen for 10 to 20 seconds and then placed in a machine for 20 seconds. The sample tube was taken out and the sample was broken into a milky white powder, and then the extraction buffer was added, and the treatment was further carried out for 1 minute or directly for subsequent extraction. The temperature of the sample was kept below -20 ° C until the buffer was added.

blade:

Take 0.2 g of plant leaves, clean the surface of the leaves, put into the sample tube, add 5 pieces of 2.0mm zirconia grinding beads, add 3 tablespoons of 1.0mm zirconia grinding beads, and cover the tube cap. The entire tube was frozen in liquid nitrogen for 10 to 20 seconds and then placed in a machine for 20 seconds. After the sample tube is taken out, the leaf is broken into a green powder, and then the extraction buffer is added, and the treatment is further carried out for 1 minute or directly for subsequent extraction. The temperature of the sample is kept below -20 ° C before the buffer is added.

Note: The Eppendorf 1.5mL Safe-Lock microcentrifuge tube (order no. 0030 120.086) must be used for the sample tube.

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