Xuesaitong Drop Pill is a new dosage form of Xuesaitong. Its main components are ginsenoside Rg1 and notoginsenoside R1 . In this experiment , the content of ginsenoside Rg1 and notoginsenoside R1 in Xuesaitong Drop Pills was determined by high performance liquid chromatography .
ã€€ 1 , instruments and reagents
ã€€ã€€ SSI's PC-3000 high performance liquid chromatography, ultraviolet spectrophotometer thermoelectric 300, ginsenoside Rg1, reference notoginsenoside R1 (China Pharmaceutical and Biological Products), Xuesaitong Pill (Lot: 20080409, 20080401, 20080402 ) The home is self-made. Methanol is chromatographically pure and water is purified water.
ã€€ã€€ 2 , methods and results
ã€€ã€€ 2.1 Detection wavelength Determination of ginsenoside Rg1 , notoginsenoside R1 reference substance amount, using methanol to prepare a solution of 20Î¼gÂ·ml-1 , scanning at a wavelength of 200 ~ 300nm , the results of ginsenoside Rg1 , notoginsenoside R1 are at 203nm Maximum absorption at the wavelength.
ã€€ã€€ 2.2 Chromatographic conditions The column was Shimadzu Kromasil C18 ( 4.6 mm Ã— 25 cm ); the mobile phase was methanol - water ( 58 : 42 ); the flow rate was 1.0 mlÂ·min-1 , and the detection wavelength was 203 nm ; the theoretical plate number was ginsenoside Rg1 . The R1 peak of notoginsenoside is not less than 10,000 , and the resolution is greater than 2.0 .
ã€€ã€€ 2.3 Determination of content
Ginsenoside Rg1 : Precisely weigh about 60mg of ginsenoside Rg1 reference substance , put it in a 50ml volumetric flask, add methanol and dilute to the mark, shake well. The precise amount of 1.0, 3.0, 5.0, 7.0, 9.0, 10.0ml respectively set 10ml volumetric flask, add methanol to the mark. 10 Î¼l of each of the above chromatographic conditions was injected into the chromatograph and measured. The results indicate that ginsenoside Rg1 was linear over the concentration peak area 0.116 ~ 1.160mg Â· ml-1 range, the regression equation Y = -99466 + 3257868X, r = 0.9992.
Notoginsenoside R1 : Precisely weigh about 60mg of the notoginsenoside R1 reference substance , put it in a 50mL volumetric flask, add methanol and dilute to the mark, shake well. The precise amount of 2.0, 3.0, 4.0, 5.0, 6.0, 7.0ml were set 10ml volumetric flask, add methanol to the mark. 10 Î¼l of each of the above chromatographic conditions was injected into the chromatograph and measured. The results show notoginsenoside R1 was in a concentration of 0.232 ~ 0.812mg Â· ml-1 range and good linear relationship between the peak area, the regression equation Y = 11772 + 2495523X, r = 0.9987.
ã€€ã€€ 3 , discussion
The auxiliary material of Xuesaitong Drop Pill is polyethylene glycol 6000 . The methanol solution of the tested polyethylene glycol 6000 has no ultraviolet absorption peak, and has no interference on the determination of the sample. The method is simple and accurate, and can be used as a method for controlling the content of ginsenoside Rg1 and notoginsenoside R1 in Xuesaitong dripping pills .
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