Principle and application of R&D cancer cell sorting kit

Like stem cells, cancer cells can proliferate indefinitely. There is increasing evidence that clonal populations of tumor cells exhibit significant heterogeneity in terms of proliferation and differentiation, and that cancer cells are produced by cells that exhibit stem cell characteristics. The hypothesis that this part of the cell promotes tumor production suggests that targeted clearance of stem cell-like cancer cell populations is a viable therapeutic strategy. In some cases, there is a lack of markers specific to cancer stem cell populations, so positive and negative markers must be used to identify these cells.

R&D Systems offers kits designed to isolate tumor-associated cell populations, including the Breast Cancer Stem Cell Isolation Kit, as well as several other kits that isolate cells expressing specific cancer-associated markers.

Breast cancer stem cell isolation

The MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit is designed to isolate a small number of human breast cancer cells that have unique capabilities to form new tumors in mice. This kit operates in a two-step process that combines negative selection and positive selection techniques. CD24low/– cells were first enriched by negative selection, while undesired CD24+ cells were labeled and removed by magnetics. CD44+ cells were subsequently isolated from the CD24low/- cell population using biotinylated human CD44 antibody, streptavidin-conjugated magnetic beads and MagCellect magnets (catalogue MAG997, or similar). The enrichment efficiency can be evaluated by staining the recovered cells with a fluorescent dye-conjugated human CD24 and CD44 detection antibody provided by the kit.

Principle of analysis

(Photo from R&D Systems)

Cancer cell sorting

In addition to the MagCellect Human CD24–CD44+ Breast Cancer Stem Cell Isolation Kit, R&D Systems offers several PlusCellect cell sorting kits that isolate positive cells for expression of specific cancer-associated markers.

Using the PlusCellect kit, the cells of interest are labeled with an analyte-specific biotinylated selection antibody, which is then bound to streptavidin-conjugated magnetic beads (MagCellect Streptavidin Ferrofluid). The labeled cells can be separated using a suitable magnetic system (MagCellect magnet), while unlabeled cells can be removed by aspirating the medium. The labeled cells are resuspended in PlusCellect buffer and the enrichment efficiency can be assessed by the fluorescent dye-bound analyte-specific detection antibody provided by each kit.

The current PlusCellect line of products includes CD44+, CXCR4+, EGF R/ErbB1+, EpCAM+, ErbB2/HER2+, HGF R/c-Met+, and Nectin-4+ cell isolation kits.

Principle of analysis

(Photo from R&D Systems)

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