ORAC and HORAC antioxidant capacity analysis

ORAC and HORAC antioxidant capacity analysis

OxiSelect ORAC/HORA Activity Assay Kit

In the detection of the oxidative stress effect, although the oxidative damage induced by ROS is widely used as an analytical index, it is also important to detect the level of cellular antioxidant capacity in vivo, cells, and extracts. The antioxidant capacity of intracellular biomacromolecules is detected at the cellular level, and can be reflected by the ORAC index (oxygen antioxidant capacity) and the HORAC index (hydroxy antioxidant capacity).

Oxygen Radical Absorbance Capacity (OCAC) and Hydroxyl Radical Antioxidant Capacity (HORAC) are two classic indicators for detecting the antioxidant capacity of biomolecules in a variety of biological samples. The detection means is to oxidize the fluorescent probe by hydrogen atom transfer reaction through hydrogen peroxide radical. In tissue cells, hydrogen peroxide radicals are produced by the oxidation of other starting radicals. During the test, the antioxidant in the sample to be tested suppresses the oxidation of the fluorescent probe to the fluorescent probe until its antioxidant capacity is lost. The remaining hydrogen peroxide radicals continue to undergo oxidation and destroy the fluorescent effect of the fluorescent probe until the inhibition time of the antioxidant and the suppression of free radical damage reach equilibrium. Therefore, the antioxidant capacity of the sample to be tested is related to the fluorescence decay curve. Specifically in the decay curve, the AUC region is often used, that is, the region below the curve. AUC is the final indicator, which is the calculated comparison of the antioxidant curve of all hydrogen peroxide radicals in the sample with the standard curve of the antioxidant vitamin E analog TroloxTM (or gallic acid).

Cell Biolabs' ORACSelectTM ORAC Activity Assay (Cat #: STA-345) and ORACSelectTM HORAC Activity Assay (Cat. No. STA-346) are reliable for rapid detection of oxy antioxidant capacity and A test kit for hydroxy antioxidant capacity. Try to analyze the antioxidant capacity of cell lysates, cytosol, serum and tissue homogenate and food extracts. Each kit was able to perform 192 analyses. The kit included a blank control, an antioxidant standard control, and the final values ​​were obtained in a high throughput format in a common microplate and within 2 hours. Easy to operate, quantitative results can be obtained due to the control provided in the kit.

Cell BioLabs' complete cellular oxidative damage assay, covering the most important DNA/RNA damage analysis products above, provides you with the optimal detection protocol. In addition to common AP site analysis and 8-OHdG/8-OHG quantitative analysis, there are also comet assays based on single cell levels and DNA double-strand break analysis. In addition to DNA/RNA damage, it also includes decene (HNE), malondialdehyde (MDA), and 8-isoprostane F2a (8-Isoprostane) ELISA assay kits during lipid peroxidation; for protein carbonylation, Protein oxidative damage detection protocols such as nitration and analysis of terminal oxidized protein products; for the detection of active oxygen groups and antioxidants, there are a variety of reagents and kits to choose from.

If you are interested in the products used in this article, please contact us at /9569/8795/4237 to discuss the oxidative stress and damage related experimental solutions, or request the latest Cell BioLabs product information.