1 Incidence

In a pig farm in Shanxi Province, on July 20, 2005, 560 piglets were introduced from a certain place in Jiangsu Province, and 400 pigs had successive diseases. He used to treat with penicillin, streptomycin, kanamycin, amoxicillin, schizophyllan, etc. The effect was poor and he died 300. After clinical diagnosis and laboratory tests, it was confirmed that the pigs were infected with Eperythrozoon suis, Streptococcus suis and Pasteurella multocida. After comprehensive treatment, the disease was quickly controlled.

2 clinical symptoms

Pigs are depressed, drowsiness, and have trouble breathing. Some pigs have white foam in their mouths, loss of appetite, body temperature from 40.5°C to 41.0°C; some chills, convulsions, unstable standing, severe limbs are swimming; some have pale skin. Or redness, especially the tip of the ear, nose, limbs, abdomen, tail, perineum purple; some constipation after diarrhea, faeces are yellow, yellow brown or gray-green water, odor or some constipation, feces was Count the plates; eventually they die, fail and die.

3 necropsy changes

A total of 3 dead pigs and 2 sick pigs were examined. Dead pig blood coagulation is poor, systemic lymph nodes are swollen and swollen, the appearance of purple black, juicy, marble-like appearance; pericardial thickening, epicardial and endocardial scattered bleeding spots; lung swelling Large, surface and section have hemorrhage; kidney surface and section have a large number of diffuse size bleeding; splenomegaly, surface and section are bleeding, showed purple black; liver enlargement, yellowish brown, surface bleeding Spots and necrosis; Gastric pylorus at a large area of ​​the hemorrhagic spot, severe loss of gastric mucosa, thickened bladder mucosa, diffuse bleeding, a large number of mucus at the throat, and bleeding points.

The liver and spleen of the diseased pig are slightly swollen. There is a bleeding point on the kidney surface. There is a lot of mucus in the throat. The intestinal tract enlarges, the intestinal wall thickens, there is a small amount of nematodes in the intestinal contents, the meningeal congestion and hemorrhage, and the brain section has small spots. Bleeding.

4 Laboratory inspection

4.1 Aseptic examination of fresh blood pressure tablets One drop of ear venous blood was placed on a glass slide, diluted with saline, and covered with a cover glass under a microscope. It was found that there were a large number of round bodies arranged around the erythrocytes, some of them were single, some were numerous, and the morphology of the infected erythrocytes changed, showing irregular shapes such as a gear shape and a polygonal shape.

4.2 Blood, heart, liver, spleen, and lung smears from dead pigs were examined by direct smear microscopy. Gram staining was performed under an ordinary light microscope. The smears of each tissue and organ were individually or in pairs. Gram-positive and small-negative bacilli. In Wright's staining, bipolar bacteria can be seen on both smears of tissues and organs.

4.3 Cultivation and biochemical characteristics observation Sterile picking heart, liver, spleen, lung disease inoculated on blood agar plate, 37 °C incubator for 24 hours, there are two kinds of colonies on the surface of agar, a needle-size colonies, smooth surface Transparent, completely transparent hemolytic rings formed around the colonies; one type of small droplet-like colonies, there is no hemolysis around it.

A single colony smear with a haemolytic ring on the tip of the needle was picked. Gram-microscopic examination revealed a large number of Gram-positive cocci. The pure broth cultured with 10% calf serum was incubated with the broth at 37°C for 24 hours. The broth was slightly turbid, and the thick filamentous bacteria floated gently when shaken. After a few hours of smear microscopy, longer chain Streptococcus (more than 10) and Streptococcus species can be seen at the same time. Biochemical tests were performed. The bacteria fermented salicin, sucrose, mannitol, maltose, sorbitol, and glucose, produced no acid, produced VP test, did not ferment inulin and raffinose, and conformed to the characteristics of streptococci.

Pick a sample of single colonies with no hemolysis in the water droplets. Microscopic examination with alkaline methylene blue shows that the bipolar bacteria stained in both poles are tested for biochemical characteristics. The oxidase and limulus reactions are positive and the urease reaction is negative. , in line with the characteristics of Pasteurella multocida.

5 diagnosis conclusion

According to the above conditions, clinical symptoms, pathological changes and laboratory tests to diagnose the disease as a combination of Streptococcus suis, Eperythrozoon and Pasteurella infection.

6 Prevention

6.1 Feeding pigs in incubation period and adding heat-clearing and detoxifying Chinese medicines to provide supportive treatment to sick pigs. The incubation period for healthy pigs is protected and the number of sick heads is minimized.

6.2 Ceftiofur for severely affected pigs, intramuscularly at 3 mg per kilogram of body weight, once daily for 3 days; bloodworm net (Benier): intramuscularly at 10 mg per kilogram of body weight, once daily for 1 day. 3 days; at the same time, add 6-methoxyuracil sodium and Vc 0.05% and 0.025% in the drinking water of the pig with reduced appetite twice a day. After 3 to 4 days of medication, the appetite of pigs recovered, symptoms were relieved for 7 days, and the condition was basically controlled.

7 Summary

7.1 The epidemic mainly involved weaned piglets, which caused great harm and caused huge economic losses. The main reason is the hot weather, long-distance transport caused by severe stress syndrome, leading to decreased resistance of piglets, so that the conditions of piglets in vivo pathogenic bacteria Streptococcus suis, Eperytria combined with Pasteurella multocida infection and disease.

7.2 Eperythrozoon of swine mainly destroys red blood cells of pigs, causing disorders of hemoglobin metabolism, leading to anemia and jaundice of the body, skin bleeding, and severe death. In addition, red blood cells are also an important type of immune cells. Once it is destroyed, it will lead to a decrease in the immune function of the pigs, which can easily cause secondary infections and increase mortality. Both Streptococcus suis and Pasteurella poricifolia are conditional pathogens, and when the body's resistance is reduced, infection can be easily caused.

7.3 Early detection of the disease, early diagnosis, early treatment. Heavy piglets can supplement iron, Vc, etc. to improve the body's resistance and facilitate the recovery of piglets.


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