In the field of analysis, due to the diversity of samples to be tested, many times, the difference between high-performance liquid chromatography and gas chromatography is often unclear. How can we quickly understand the difference between the two? What? Here, Shandong Luchuang Analytical Instruments Co., Ltd. gas chromatograph manufacturers technical staff to popularize this knowledge for us.
1. Analysis of similarities and differences
Gas Chromatography Separation (Gas Chromatography): Adapt to sample analysis that is easy to vaporize, has good thermal stability, and has a relatively low boiling point. However, it is not suitable for sample analysis of high boiling point, poor volatility, poor thermal stability, ionic and high polymer; the disadvantage of chromatography is that it is difficult to characterize the unknown, if there is no known pure standard sample or known pure The chromatogram of the sample makes it difficult to determine what a chromatographic peak represents; the quantitative analysis of components is an advantage.
High Performance Liquid Chromatograph Separation (Liquid Chromatography): A sample or organic medium solution suitable for solution after dissolution, independent of the volatility and thermal stability of the sample, more than 70% organic The compounds can be analyzed by high performance liquid chromatography, especially the separation and analysis of high boiling point, macromolecule, poor volatility, strong polarity, and poor thermal stability, showing advantages.
2. Similarities and differences of mobile phases
Gas Chromatograph: The mobile phase is inert, that is, the flow into the column is gaseous, and the gas component has no affinity with the mobile phase, and only interacts with the stationary phase.
Liquid Chromatograph: The mobile phase is a liquid, and the mobile phase and the components have a synergistic force, which can improve the selectivity of the column, improve the resolution, and play a positive role in the separation. When two or more different ratios are selected, Liquid as a mobile phase can also increase separation selectivity.
3. Similarities and differences in operating conditions
Gas Chromatograph (GC) : Analytical operation is required under heating conditions. If different inlet temperature, detector temperature, and column temperature must be set. For some high boiling samples, a thermal cracker is required for injection.
Liquid Chromatograph (HPLC): For most samples analyzed at normal room temperature; the mobile phase is high pressure (large liquid viscosity, small peak broadening).
These are the similarities and differences in gas chromatography and high performance liquid chromatograph description, I believe we have now clearly understand their respective roles and features, and if you have any questions, you can contact the telephone or log Shandong Lu Chong online counseling staff.