The broodstock for flea breeding should be carefully selected. Female quails should have a body length of at least 35 cm, weigh between 50 to 150 grams, and show signs of a swollen belly with a zona pellucida, indicating readiness for reproduction. Their cloaca should appear bloated, and their granulose should be well-developed. For males, they should be longer, at least 55 cm in length, weigh over 150 grams, and display blood-streaked markings on the ventral side. When gently pressing their abdomen, a transparent fluid should be released. A microscopic examination can confirm the presence of viable spermatozoa. It is recommended to pair one male with 2 to 3 females for optimal breeding results.
To enhance fertility, oxytocin can be used to stimulate ovulation, increase water exchange, regulate breeding density, and provide high-quality feed. Alternatively, a single thoracic injection of LRH-A (Luteinizing Hormone-Releasing Hormone analog) can be administered. The dosage for females is 10 micrograms per 50 grams of body weight, while males receive 15 to 25 micrograms per tail. Males should be injected 24 hours before females, and the injection depth should not exceed 0.5 cm to avoid damage.
For artificial insemination, one hand holds the female quail in a towel, while the other gently presses the abdomen backward. If the cloaca becomes blocked, it may need to be carefully cut open, and then the eggs can be manually squeezed out. The collected eggs are placed into a sterile container or basin. To prepare the semen, select a male raccoon, euthanize it, remove the testes, crush them, and pass the mixture through a 100-mesh sieve to extract the seminal fluid. This is then added to the egg container and stirred for 5 minutes. Any remaining tissue and blood residue should be washed away with clean water. At least two males should be used for insemination, regardless of the number of eggs, to ensure successful fertilization.
During incubation, maintain a consistent water temperature, with an incubation depth of approximately 10 cm. Water should be changed regularly, but the temperature difference should not exceed 4°C. Higher temperatures will reduce incubation time. Cement tanks are ideal for raising seedlings. Apply appropriate amounts of manure to support silkworm cultivation, and introduce aquatic plants like water hyacinth on the water surface to provide natural cover. Seedlings should be stocked at a density of around 200 per square meter. After 1 to 2 months, when the seedlings grow to about 10 cm in length, they can be transferred to a breeding pond for further growth and feeding.
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