First, the choice of broodstock 1. The source of the broodstock: The broodstock used for artificial breeding, first, from the natural waters to catch the weight of 0.25 ~ 0.75 kg, a total length of 21 to 31 centimeters, after the return of 30 to 50 days The short-term cultivation can be matured in the same year and oxytocin production; the second is to cultivate broodstock in the pond, and mature after careful feeding and feeding management. 2, broodstock male and female identification of the rear of the anus of the carp has a white cylindrical small protrusions (ie, reproductive processes), in this reproductive process, the female has two openings - reproductive hole and vent hole, opening in the top of the reproductive process . Based on this appearance characteristic, males and females of carp can be identified whether in the reproductive season or in the non-reproductive season. In addition, when the carp is sexually mature, especially in the breeding period, the female mandibular front has a rounded shape, not more than the upper jaw; the male mandible has a sharp angle at the front end of the mandible, which is more than the upper jaw. 3, the choice of mature broodstock mature broodstock appearance characteristics are: female abdomen is more inflated, gently press the abdomen by hand, soft and flexible, the outline of the ovary is clear, the abdomen midline concave, after the fall of the ovary has a moving shape; male reproductive hole relaxation , light pressure on the abdomen with milky semen outflow, and semen into the water immediately dispersed. (1) Kind of aphrodisiac. The aphrodisiac used for the artificial propagation of squid mainly includes three kinds: catfish, carp pituitary, chorionic stimulating hormone and luteinizing hormone releasing hormone analogue. (2) Injection method and dosage. General use of body cavity injection, in the pectoral fin base without fins recessed part, the needle head toward the fish's head and the body axis into a 45-degree angle, piercing the body cavity, slowly into the liquid. There are two injections, one injection and two injections. One injection. If the pituitary gland is used alone, then the female injection volume is 14 to 16 mg/kg; the chorionic gonadotropin and the pituitary gland are mixed, and the female injection volume is 2 mg/kg for the pituitary gland and 3 to 6 mg for the chorionic gonadotropin . If luteinizing hormone-releasing hormone analogues are used, the injection dose differs depending on the size of the fish, 200 mg/kg for females weighing more than 3 kg, 200 μg/kg for 1 kg to 2 kg, and 1 for body weight. Injections of 400 micrograms/kilogram of females below the kilogram. The male fish was injected at half the dose of the above female. Two injections. Two injections generally use the pituitary gland better. The first dose was 0.8-1.6 mg/kg for females and half for males. The second dose was 10 to 15 milligrams per kilogram for females and half for males. The interval between the first injection and the second injection is generally 6-8 hours. Generally two injections have a better ovulation effect. Notes: The fins of the carp are profitable and hard. When catching carp, use the thumb and index finger to pinch the mandible of the carp and raise the fish. Then use a gauze and towel to wrap the fish and leave the injection site. Injectable. This method not only does not harm the fish, but also ensures that the injection personnel are not punctured by the fins. 4. The optimum temperature for spawning of natural spawning carps is 25°C～31°C. In the breeding season, after the mature broodstock is injected with the scavenger, the ratio of male and female can be paired and spawned in the spawning pool to spawn by themselves. Generally, males should be slightly more than females, and the ratio of male to female is 1:1.5. Under the stimulation of aphrodisiac, coupled with the stimulation of flushing water in the spawning tank, the eel excitement phenomenon will appear after a period of time. At the beginning, several fishes clustered close together and swim in the top water. Then, the males chased the females and used their bodies to violently rub the female abdomen. When the estrus culminated, the females produced eggs, and at the same time The fish row is fine, the egg is combined with sperm and fertilized (fertilized egg). Fertilized eggs hatch at the right temperature. If there is no spawning pool, the broodstock injected with the oxytocide may also be placed in a sieve basket. After a certain period of time, the broodstock can estrus and spawn on its own. After the broodstock has produced its eggs, the broodstock can be picked up and removed, and then the eggs in the box can be pooled together, and they can be placed in the basin or other utensils and transferred to the incubator for hatching. The length of time from when the broodstock was injected to aphrodisiac to the beginning of estrus and spawning was related to the water temperature and the number of injections. When the water temperature is high, estrus and spawning time are short, and when the water temperature is low, it takes a long time. Ejaculation time of two injections (from the second injection) is shorter than one injection. Therefore, the production of fish eggs can be properly arranged according to the water temperature. The natural spawning method is relatively simple and easy. The broodstock is lightly injured, but the fertilization rate is lower than that of artificial insemination. 5, artificial insemination (1) When the broodstock has been estrus, but has not yet reached the climax (ie, 15 minutes after the start of estrus estrus), immediately pulled the net to catch the broodstock, the female abdomen upward, gently pressing the abdomen with egg outflow At the time, grab the genital hole and clean the fish surface water, then turn the fish belly down and let the eggs flow into the pre-cleaned porcelain basin. At the same time, immediately add the male semen and stir it with feathers for 1-2 minutes. The eggs are thoroughly mixed, then a small amount of fresh water is added, and the mixture is stirred. After standing for 1 minute, it can be placed in an incubation tank for incubation. (2) Artificial incubation requires a certain amount of water flow during incubation. Normal hatching tanks (capacity about 200 liters) or hatching tanks for hatching fish eggs are used for hatching. Incubation water temperature is 20 °C ~ 30 °C water capacity of 6 mg / liter or more, the flow rate in the hatching tank should be larger than when hatching fish eggs. After 3 to 4 days, fry can be hatched.
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