The easiest way is to take a large oil drum, remove the lid, place it on a stove supported by a brick, add more than 20cm of water to the drum, place a fence, and place it near the bottom of the fence. A 2.5cm diameter hole is opened in the barrel wall, and a piece of “Lâ€-shaped hollow steel pipe is welded and the mouth is facing upwards, so that the pot can be replenished in time during the sterilization process. Usually plugged with a cork, unplug it when you add water. Place the packed bacteria bags vertically in the barrel, and leave gaps between the bags to facilitate steam circulation.
After the bacterial bag is full, a plastic film is added to the surface of the barrel to form a woven bag. The old inner tube of the bicycle is used to fasten it over the wall of the barrel, and then the fire is heated under the barrel. At the start of the fire, the fire broke out. When the plastic film at the top of the drum bulged, it was timed (100 °C in the sterilizer), and it continued to burn for 14 to 16 hours. Then the heat sterilization was stopped. During the sterilization process, the fire should be fired in the middle after the pre-attack, that is, when the fire is started, when it reaches 100°C, it is continued for another hour, and then it is kept at 100°C. Within 1 hour before the end, it is still burnt with fire. .
After sterilization, when the temperature of the stove falls below 70°C, the pan can be panned. The sterilized bag is placed in a clean room and cooled. When the bag temperature drops below 30°C, it can be inoculated under aseptic conditions.
Creation of aseptic conditions: The easiest method at present is to use plastic film to weld the same inoculation net as the net, hang it up in four corners, and move the sterilized material bag and bacteria (using 75% alcohol to scrub the outer wall) to the inoculation In the account, the bottom of the account is rolled inwards and pressed tightly with a material bag. The door is affixed with two-sided stickers, and "mushroom protection number 1" or 5 mg of potassium permanganate plus 10 grams of formaldehyde per cubic meter of space is used. Disinfection was carried out. The disinfectant was placed in a porcelain dish and ignited for 30 minutes after being sterilized by fumigation and then inoculated. The vaccinator should change the clean clothes before entering the inoculation account. Inoculation tools such as inoculation shovel (inoculated shovel can be made of stainless steel bar or bicycle spokes, etc.) first soaked in 75% alcohol solution for 5 minutes, then the bacteria species (bags or bottles) The old strains were excavated. The sterilized material bags were opened and the strains were rapidly transferred to them. The lines were sealed again and then another bag was used until the inoculation was completed. During the inoculation, avoid walking around freely, and do not enter and leave at random. Otherwise, it is very easy to infect the bacteria and cause losses.
(3) Fungal management: According to the physiological characteristics of Pleurotus eryngii, although it can grow below 35°C, it should be controlled at the optimum temperature of 24°C～26°C. Insulation can be adopted when the temperature is low, and the temperature should be reduced when it is high. 7 to 10 days inspection of the bag once, found that green, red, black and other mold should be singled out for treatment. The indoor air humidity of the bacteria-producing bacteria should be maintained at about 60%. If the humidity is high, proper ventilation can be performed to adjust it. Generally, the culture can be carried out for 30 to 35 days, and the mycelium of Pleurotus eryngii can be covered with a bag. The draft is provided by Han Chaozheng, Institute of Edible Fungus, Binhai County, Jiangsu Province, China. Tel: 0515-4103012 13851113822
After the bacterial bag is full, a plastic film is added to the surface of the barrel to form a woven bag. The old inner tube of the bicycle is used to fasten it over the wall of the barrel, and then the fire is heated under the barrel. At the start of the fire, the fire broke out. When the plastic film at the top of the drum bulged, it was timed (100 °C in the sterilizer), and it continued to burn for 14 to 16 hours. Then the heat sterilization was stopped. During the sterilization process, the fire should be fired in the middle after the pre-attack, that is, when the fire is started, when it reaches 100°C, it is continued for another hour, and then it is kept at 100°C. Within 1 hour before the end, it is still burnt with fire. .
After sterilization, when the temperature of the stove falls below 70°C, the pan can be panned. The sterilized bag is placed in a clean room and cooled. When the bag temperature drops below 30°C, it can be inoculated under aseptic conditions.
Creation of aseptic conditions: The easiest method at present is to use plastic film to weld the same inoculation net as the net, hang it up in four corners, and move the sterilized material bag and bacteria (using 75% alcohol to scrub the outer wall) to the inoculation In the account, the bottom of the account is rolled inwards and pressed tightly with a material bag. The door is affixed with two-sided stickers, and "mushroom protection number 1" or 5 mg of potassium permanganate plus 10 grams of formaldehyde per cubic meter of space is used. Disinfection was carried out. The disinfectant was placed in a porcelain dish and ignited for 30 minutes after being sterilized by fumigation and then inoculated. The vaccinator should change the clean clothes before entering the inoculation account. Inoculation tools such as inoculation shovel (inoculated shovel can be made of stainless steel bar or bicycle spokes, etc.) first soaked in 75% alcohol solution for 5 minutes, then the bacteria species (bags or bottles) The old strains were excavated. The sterilized material bags were opened and the strains were rapidly transferred to them. The lines were sealed again and then another bag was used until the inoculation was completed. During the inoculation, avoid walking around freely, and do not enter and leave at random. Otherwise, it is very easy to infect the bacteria and cause losses.
(3) Fungal management: According to the physiological characteristics of Pleurotus eryngii, although it can grow below 35°C, it should be controlled at the optimum temperature of 24°C～26°C. Insulation can be adopted when the temperature is low, and the temperature should be reduced when it is high. 7 to 10 days inspection of the bag once, found that green, red, black and other mold should be singled out for treatment. The indoor air humidity of the bacteria-producing bacteria should be maintained at about 60%. If the humidity is high, proper ventilation can be performed to adjust it. Generally, the culture can be carried out for 30 to 35 days, and the mycelium of Pleurotus eryngii can be covered with a bag. The draft is provided by Han Chaozheng, Institute of Edible Fungus, Binhai County, Jiangsu Province, China. Tel: 0515-4103012 13851113822
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